Jul 28, 2025
Version 1
LRRK2-mutant microglia and neuromelanin synergize to drive dopaminergic neurodegeneration in an iPSCbased Parkinson’s disease model V.1
- 1VHIR-CIBERNED-ASAP
- Vilalab Public
Collection Citation: Miquel Vila 2025. LRRK2-mutant microglia and neuromelanin synergize to drive dopaminergic neurodegeneration in an iPSCbased Parkinson’s disease model. protocols.io https://dx.doi.org/10.17504/protocols.io.q26g7n8e9lwz/v1Version created by Miquel Vila
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: July 28, 2025
Last Modified: July 28, 2025
Collection Integer ID: 223409
Keywords: ipscbased parkinson, dopaminergic neurodegeneration, mutant microglia, neuromelanin, article lrrk2
Funders Acknowledgements:
ASAP
Grant ID: ASAP-020505
Abstract
Collection of protocols included in article LRRK2-mutant microglia and neuromelanin synergize to drive dopaminergic neurodegeneration in an iPSCbased Parkinson’s disease model
Files
Protocol
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iPSC derived microglia (hMG) culture
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Miquel VilaVHIR-CIBERNED-ASAP
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iPSC-derived vmDAn
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Miquel VilaVHIR-CIBERNED-ASAP
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iPSC-derived organoid generation
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Miquel VilaVHIR-CIBERNED-ASAP
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2D and 3D Neuron/Microglia co-culture systems
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Miquel VilaVHIR-CIBERNED-ASAP
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Immunocytochemistry
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Miquel VilaVHIR-CIBERNED-ASAP
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Flow Cytometry
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Miquel VilaVHIR-CIBERNED-ASAP
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Gene expression analysis
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Miquel VilaVHIR-CIBERNED-ASAP
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Cytokine Array
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Miquel VilaVHIR-CIBERNED-ASAP
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Synaptosome extraction
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Miquel VilaVHIR-CIBERNED-ASAP
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Western Blot
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Miquel VilaVHIR-CIBERNED-ASAP
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pHrodo labeled synaptosome phagocytic assay
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Miquel VilaVHIR-CIBERNED-ASAP
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Neuromelanin phagocytosis assay
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Miquel VilaVHIR-CIBERNED-ASAP
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Motility analysis of iPSC-derived microglia
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Miquel VilaVHIR-CIBERNED-ASAP
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Intracellular ROS measurement in iPSC-derived microglia
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Miquel VilaVHIR-CIBERNED-ASAP
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IBA1 Immunochemistry on human paraffin sections
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Created by
Miquel VilaVHIR-CIBERNED-ASAP
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Intracellular neuromelanin quantification
Version 1
Created by
Miquel VilaVHIR-CIBERNED-ASAP