Jul 28, 2025

Public workspaceLRRK2-mutant microglia and neuromelanin synergize to drive dopaminergic neurodegeneration in an iPSCbased Parkinson’s disease model V.1

  • Miquel Vila1
  • 1VHIR-CIBERNED-ASAP
  • Vilalab Public
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Collection CitationMiquel Vila 2025. LRRK2-mutant microglia and neuromelanin synergize to drive dopaminergic neurodegeneration in an iPSCbased Parkinson’s disease model. protocols.io https://dx.doi.org/10.17504/protocols.io.q26g7n8e9lwz/v1Version created by Miquel Vila
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: July 28, 2025
Last Modified: July 28, 2025
Collection Integer ID: 223409
Keywords: ipscbased parkinson, dopaminergic neurodegeneration, mutant microglia, neuromelanin, article lrrk2
Funders Acknowledgements:
ASAP
Grant ID: ASAP-020505
Abstract
Collection of protocols included in article LRRK2-mutant microglia and neuromelanin synergize to drive dopaminergic neurodegeneration in an iPSCbased Parkinson’s disease model
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Protocol
Name
iPSC derived microglia (hMG) culture
Version 1
, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP
Protocol
Name
iPSC-derived vmDAn
Version 1
, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP
Protocol
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iPSC-derived organoid generation
Version 1
, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP
Protocol
Name
2D and 3D Neuron/Microglia co-culture systems
Version 1
, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP
Protocol
Name
Immunocytochemistry
Version 1
, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP
Protocol
Name
Flow Cytometry
Version 1
, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP
Protocol
Name
Gene expression analysis
Version 1
, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP
Protocol
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Cytokine Array
Version 1
, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP
Protocol
Name
Synaptosome extraction
Version 1
, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP
Protocol
Name
Western Blot
Version 1
, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP
Protocol
Name
pHrodo labeled synaptosome phagocytic assay
Version 1
, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP
Protocol
Name
Neuromelanin phagocytosis assay
Version 1
, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP
Protocol
Name
Motility analysis of iPSC-derived microglia
Version 1
, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP
Protocol
Name
Intracellular ROS measurement in iPSC-derived microglia
Version 1
, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP
Protocol
Name
IBA1 Immunochemistry on human paraffin sections
Version 1
, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP
Protocol
Name
Intracellular neuromelanin quantification
Version 1
, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP