Sep 09, 2021
Longmire lysis buffer
- Abigail Wells1,
- Linda Park1
- 1NWFSC-NOAA
External link: https://doi.org/10.1371/journal.pone.0257773
Protocol Citation: Abigail Wells, Linda Park 2021. Longmire lysis buffer. protocols.io https://dx.doi.org/10.17504/protocols.io.bx4xpqxn
Manuscript citation:
Use of "lysis buffer" in DNA isolation and its implications for museum collections J. Longmire, M. Maltbie and R. J. Baker Occasional Papers Museum of Texas Tech University 1997 Vol. 163 DOI: 10.5962/bhl.title.143318
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
Created: September 09, 2021
Last Modified: September 09, 2021
Protocol Integer ID: 53111
Abstract
Lysis buffer recipe (Longmire et al 1997):
To make 1 liter
Materials
H2O
1 Molarity (M) Tris-HCL, 8
0.5 Molarity (M) EDTA, 8
5 Molarity (M) NaCl
20 Mass Percent SDS (w/v)
To make 1 L of Longmire
To make 1 L of Longmire
975 ml double-distilled water
100 ml of 1 M Tris-HCL, pH 8.0
200 ml of 0.5 M EDTA, pH8.0
2 ml of 5 M NaCl
25 ml of 20% SDS (w/v) Filter the buffer with an autofill PES bottle top filtration device (sterile 500ml, 0.22 µm)