Jan 21, 2020
Longitudinal Analysis of C elegans (L4, YA D2 & YA D5)
- 1Imperial College London
- Behavioural Genomics
Protocol Citation: Priota Islam 2020. Longitudinal Analysis of C elegans (L4, YA D2 & YA D5). protocols.io https://dx.doi.org/10.17504/protocols.io.bbk2ikye
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: January 21, 2020
Last Modified: January 21, 2020
Protocol Integer ID: 32122
Bleach synchronize the worms following the protocol Bleach synchronisation of C. elegans
Refeed the arrested L1s 2 days prior to tracking (Refeed per strain on 3 plates)
Prepare the imaging plates by seeding with 50ul OP50 and leave to dry on bench top/hood
Image the strains at the following stages: L4, Day 2 and Day 5.
On the day of imaging, pick 5 worms per imaging plate and place on the Phenix rig to acclimatize for 30mins followed by imaging for 15minutes
Discard the plates post imaging
Note:
On D2 of the tracking, pick around 40 worms from each strain onto 2 new plates for D5 tracking, otherwise the plate will be too crowded, and it will be difficult to differentiate the age.