Oct 14, 2020
Long-Term Storage of Bacteria
This protocol is a draft, published without a DOI.
- 1Chung Shan Medical University
Protocol Citation: Huan Jui Chang, iGEM-CSMU 2020. Long-Term Storage of Bacteria. protocols.io https://protocols.io/view/long-term-storage-of-bacteria-bh48j8zw
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: July 01, 2020
Last Modified: October 14, 2020
Protocol Integer ID: 38784
Abstract
This protocol is suitable for preparing bacterial storage for -80 degree Celsius with glyceral as the solvent. It is usually performed after the overnight incubation of transformed bacteria that are ready for plasmid extraction.
Guidelines
The concentration of glycerol is optimized for around 17% in this protocol.
Safety warnings
Make sure that all the steps should be operated in laminar flow.
Before start
Follow the "preparation" section of protocol.
Preparation
Preparation
Turn on the UV light of the laminar flow for 00:10:00 to 00:15:00 in order to sterilize it.
Protocol
Protocol
Add 300 μL of 50% glycerol into an eppendorf.
Add 600 μL of bacterial media prepared in a procedure like "Bacterial Culture for Plasmid Extraction" to an eppendorf.
Store the eppendorf under -80 degree Celsius.