Aug 23, 2024

Liquid Growth Medium - Yeast

DOI
https://dx.doi.org/10.17504/protocols.io.5qpvokr2bl4o/v1
Liquid Growth Medium - Yeast
  • 1UMass Chan Medical School, RNA Therapeutics Institute, Worcester, MA, USA;
  • 2University of Alberta, Department of Cell Biology, Edmonton, AB, Canada;
  • 3University of California, Department of Viticulture and Enology, Davis, CA, USA
Mathias Hammer
Icon indicating open access to content
QR code linking to this content
DOI: https://dx.doi.org/10.17504/protocols.io.5qpvokr2bl4o/v1
Protocol Citation: Mathias Hammer, Ammeret Rossouw, Azra Lari, Ben Montpetit, David Grunwald 2024. Liquid Growth Medium - Yeast. protocols.io https://dx.doi.org/10.17504/protocols.io.5qpvokr2bl4o/v1
License: This is an open access  protocol  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: May 16, 2024
Last Modified: August 23, 2024
Protocol  Integer ID: 99988
Keywords: yeast liquid grow medium, yeast medium, liquid culture medium for saccharomyces cerevisiae, liquid culture medium, liquid growth medium, saccharomyces cerevisiae, yeast this protocol, yeast
Funders Acknowledgements:
NSF
Grant ID: 1917206
Disclaimer
DISCLAIMER – FOR INFORMATIONAL PURPOSES ONLY; USE AT YOUR OWN RISK

The protocol content here is for informational purposes only and does not constitute legal, medical, clinical, or safety advice, or otherwise; content added to protocols.io is not peer reviewed and may not have undergone a formal approval of any kind. Information presented in this protocol should not substitute for independent professional judgment, advice, diagnosis, or treatment. Any action you take or refrain from taking using or relying upon the information presented here is strictly at your own risk. You agree that neither the Company nor any of the authors, contributors, administrators, or anyone else associated with protocols.io, can be held responsible for your use of the information contained in or linked to this protocol or any of our Sites/Apps and Services.
Abstract
This protocol describes the steps to prepare liquid culture medium for Saccharomyces cerevisiae.
Materials
SC-Ura Powder
Sunrise Science Products
Cat#: 1306-030
Lot#: 23K3083
Exp: 10/2027

Yeast Nitrogen Base Without Amino Acids
Sigma Life Science
Cat#: Y0626-250G
Lot#: SLBG0555V

Glucose
Sunrise Science Products
Cat#: 1907-1kg
Lot#: 3A0036

Deionized Water

Equipment:

500 ml laboratory bottle with screw cap
1ml pipette
50 ml pipette
stirring hot plate
magnetic stirring bar
micro scales
autoclave
Before start
Have the following solutions premixed:

Glucose 20% 500 ml solution:
Concentration: 200 g/l
mix 100 g Glucose in 500 ml deionized water (ddH2O)

Optional:
SC-xx 10x 100ml solution:
Concentration: 19.2 g/l
mix 1.92 g into 100 ml ddH2O

YNB 20x 100ml solution:
Concentration: 134.4 g/l
mix 13.44 g into 100 ml ddH2O
Compound medium for autoclave
Step case

Medium preparation with pre-resolved components
6 steps

This version of the protocol shows the preparation of the medium from SC-XX 10x and YNB 20x solutions.
Fill a 500 ml flask with 325 mL ddH2O.
Add a magnetic stirring bar and place the flask on a stirring hot plate.
Add 25 mL YNB 20x solution (Yeast Nitrogen Base with Ammonium Sulfate without Amino Acids).
Add 100 mL SC-XX 10x solution.
Note
In regard to cover all optional dropout media the amino acid base holds the notification -xx, where xx stand for the amino acid(s) that is as selection factor, missing in the medium.

Autoclave for 00:15:00 at 121 °C .
Note
Remove the stirring bar before going to autoclave.

When the medium cooled down to around 80 °C add 50 mL sterile Glucose 20%.

Note
The medium can be store at the bench for 2 to 3 months.