In this protocol, lipids in miroalgae is extracted with 2 ml Folch solvent (2:1 chloroform-methanol v\/v) after frozen and thawed with the addition of 100 ul water. Non-lipid substances are removed by filtration. Filtrate is then mixed with 0.88% potassium chloride solution to form a biphase system. The lower phase with extracted lipids are collected and dried under N2 gas flow. The residue is dissolved in 5 ml chloroform and stored under -80 \u00baC.