This protocol is a two-part lipid extraction for labyrinthulomycetes. The only strain we have tried this protocol on so far is Aurantiochytrium limacinum ATCC MYA-1381.This protocol was modified from: Higgins, B.T., A. Thornton-Dunwoody, J.M. Labavitch, J.S. VanderGheynst. 2014. Microplate assay for quantitation of neutral lipids in extracts from microalgae. Anal. Biochem. 465: 81-89.