Dec 22, 2025
LHb recordings
- Asa Mackenzie1
- 1Lund University
Protocol Citation: Asa Mackenzie 2025. LHb recordings. protocols.io https://dx.doi.org/10.17504/protocols.io.14egn1xzqv5d/v1
Manuscript citation:
Serra GP, Guillaumin A, Vlcek B, Delgado-Zabalza L, Ricci A, Rubino E, Dumas S, Baufreton J, Georges F, Wallén-Mackenzie Å. A role for the subthalamic nucleus in aversive learning. Cell Rep. 2023 Nov 28;42(11):113328. doi: 10.1016/j.celrep.2023.113328. Epub 2023 Nov 4. PMID: 37925641.
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: December 22, 2025
Last Modified: April 21, 2026
Protocol Integer ID: 235675
Keywords: ASAPCRN, lhb recordings lhb recording
Funders Acknowledgements:
Aligning Science Across Parkinsons
Grant ID: ASAP-020600
Abstract
LHb recordings
LHb recordings
An optic fiber and a recording pipette filled with either 2% pontamine sky blue or 2% neurobiotin were respectively positioned in the
STN and LHb.
For each LHb neurons, a PSTH was recorded upon STN optogenetic stimulation (PSTH 0.5 Hz, 5 ms pulses, 5–8 mW)
for at least 100 s.
Neurons are considered as excited during the PSTH protocol when, following the light pulses centered on 0, the number of spikes/5ms bin is higher than the baseline (500 ms–0 ms) plus two times the standard deviation.
Injection of neurobiotin (2% neurobiotin in 0.5 M acetate sodium) by juxtacellular iontophoresis was performed in some cases for precise spatial identification of excited neurons.