Mar 05, 2025
Levodopa treatment for low-dose 6-OHDA-treated mice
Forked from Levodopa-induced dyskinesia mouse model
- Beatriz Nielsen1
- 1University of Colorado Anschutz Medical Campus
Protocol Citation: Beatriz Nielsen 2025. Levodopa treatment for low-dose 6-OHDA-treated mice. protocols.io https://dx.doi.org/10.17504/protocols.io.4r3l29dpjv1y/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: March 01, 2025
Last Modified: March 05, 2025
Protocol Integer ID: 123643
Keywords: ASAPCRN, week levodopa treatment for mice, levodopa treatment, week levodopa treatment, dopamine, treated mice, treatment, mice this protocol, mice
Abstract
This protocol describes a one-week levodopa treatment for mice partially depleted of dopamine.
Troubleshooting
Levodopa treatment
Generate unilaterally low-dose 6-OHDA-lesioned mice (protocol linked below):
3 weeks after low-dose 6-OHDA injections, verify unilateral partial dopamine depletion by assessing forelimb use assymetry in the cylinder test (protocol linked below).
Start daily intraperitoneal (i.p.) injections of 2 mg/kg of L-DOPA (3,4-dihydroxy-L-phenylalanine) plus 12 mg/kg of benserazide hydrochloride dissolved in sterile saline for 7-8 days.
Acute brain slices for electrophysiology recordings (protocol linked below) must be prepared 30min – 1 h after the last L-DOPA administration.