Mar 05, 2025
Levodopa treatment for low-dose 6-OHDA-treated mice
Forked from Levodopa-induced dyskinesia mouse model
- 1University of Colorado Anschutz Medical Campus
Protocol Citation: Beatriz E Nielsen 2025. Levodopa treatment for low-dose 6-OHDA-treated mice. protocols.io https://dx.doi.org/10.17504/protocols.io.4r3l29dpjv1y/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: March 02, 2025
Last Modified: March 05, 2025
Protocol Integer ID: 123643
Keywords: ASAPCRN
Abstract
This protocol describes a one-week levodopa treatment for mice partially depleted of dopamine.
Levodopa treatment
Levodopa treatment
Generate unilaterally low-dose 6-OHDA-lesioned mice (protocol linked below):
Protocol
NAME
6-OHDA mouse model of Parkinson's diseaseCREATED BY
Beatriz E Nielsen
3 weeks after low-dose 6-OHDA injections, verify unilateral partial dopamine depletion by assessing forelimb use assymetry in the cylinder test (protocol linked below).
Protocol
NAME
Motor behavioral assessmentCREATED BY
Beatriz E Nielsen
Start daily intraperitoneal (i.p.) injections of 2 mg/kg of L-DOPA (3,4-dihydroxy-L-phenylalanine) plus 12 mg/kg of benserazide hydrochloride dissolved in sterile saline for 7-8 days.
Acute brain slices for electrophysiology recordings (protocol linked below) must be prepared 30min – 1 h after the last L-DOPA administration.
Protocol
NAME
Acute Brain Slices CREATED BY
Kelsey Barcomb