Jun 30, 2025

Lentiviral transduction

Nature Communications
DOI
https://dx.doi.org/10.17504/protocols.io.rm7vz9mb4gx1/v1
  • 1Department of Physiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, 19104;
  • 2Aligning Science Across Parkinson’s (ASAP) Collaborative Research Network, Chevy Chase, MD, 20815
  • University of Pennsylvania
Bishal Basak
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DOI: https://dx.doi.org/10.17504/protocols.io.rm7vz9mb4gx1/v1
External link: https://doi.org/10.1038/s41467-025-62379-5
Protocol Citation: Bishal Basak, Erika Holzbaur 2025. Lentiviral transduction. protocols.io https://dx.doi.org/10.17504/protocols.io.rm7vz9mb4gx1/v1
Manuscript citation:
Basak, B., Holzbaur, E.L.F. Mitochondrial damage triggers the concerted degradation of negative regulators of neuronal autophagy. Nat Commun 16, 7367 (2025). https://doi.org/10.1038/s41467-025-62379-5
License: This is an open access  protocol  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: June 30, 2025
Last Modified: June 30, 2025
Protocol  Integer ID: 221326
Keywords: lentiviral transduction lentiviral transduction of primary neuron, lentiviral transduction lentiviral transduction, primary neuron, transduction
Funders Acknowledgements:
ASAP
Grant ID: ASAP-000350
Abstract
Lentiviral transduction of primary neurons
Plate neurons
Plate neurons in a dish at days in vitro (DIV) 0
Infection with virus
At DIV=1 infect neurons with lentiviral particles at MOI=9
When neurons are mature (DIV>=6), remove media containing virus, and treat neurons based on experimental needs
Post treatment lyse samples in lysis buffer to be used for western blotting later