We use this protocol to extract and study acylsugars from glandular trichomes present on the surface of Solanum lycopersicum and Solanum pennellii plants as described in our manuscript "Evolution of metabolic novelty: a trichome-expressed invertase creates specialized metabolic diversity in wild tomato" (doi: https://doi.org/10.1101/502971).While this method is optimized for acylsugar extraction, it can be tweaked to use for other metabolites on the leaf surface.Below is a literature that is highly useful for acyslugar identification:Comparative structural profiling of trichome specialized metabolites in tomato (Solanum lycopersicum) and S. habrochaites: acylsugar profiles revealed by UHPLC/MS and NMR (doi: https://doi.org/10.1007/s11306-013-0585-y)While this method is optimized for acylsugar extraction, it can be tweaked to use for other metabolites on the leaf surface.Below is a literature that is highly useful for acyslugar identification:Comparative structural profiling of trichome specialized metabolites in tomato (Solanum lycopersicum) and S. habrochaites: acylsugar profiles revealed by UHPLC/MS and NMR (doi: https://doi.org/10.1007/s11306-013-0585-y)Below is a literature that is highly useful for acyslugar identification:Comparative structural profiling of trichome specialized metabolites in tomato (Solanum lycopersicum) and S. habrochaites: acylsugar profiles revealed by UHPLC/MS and NMR (doi: https://doi.org/10.1007/s11306-013-0585-y)