Oct 10, 2025
LBM Ventilation Protocol
- Christopher LeBoa1,
- Nadia Rimi2,
- Shohanur Rahman2,
- Khaled Saifullah2,
- Layla Kwong1,
- Ajay Pillaisetti1
- 1UC Berkeley;
- 2icddr,b
Protocol Citation: Christopher LeBoa, Nadia Rimi, Shohanur Rahman, Khaled Saifullah, Layla Kwong, Ajay Pillaisetti 2025. LBM Ventilation Protocol . protocols.io https://dx.doi.org/10.17504/protocols.io.ewov11x2ovr2/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We used this protocol. It works but the question on how to utilize it in different types of spaces (without walls) is an open question of if it provides any accuracy
Created: October 09, 2025
Last Modified: October 10, 2025
Protocol Integer ID: 229469
Keywords: lbm ventilation protocol, avian influenza in live poultry market, ventilation, relationship between ventilation, ventilation components of work, ventilation component, avian influenza, live poultry market
Funders Acknowledgements:
National Institutes of Health
Grant ID: D43TW010540
Abstract
This is the protocol used for the ventilation components of work looking at the relationship between ventilation and airbore avian influenza in live poultry markets in Bangladesh
Materials
- Fire extinguisher
- 5 x Aranet4 CO2 monitors
- Tablet with Aranet4 application (Pair sensors with the device using this protocol)
- Wire or other mechanism for attaching Aranet4 to wall
- Cardboard for mixing air
Troubleshooting
Procedure
Position the Aranet4 monitors in the shop
Slaughtering area
Defeathering area
Shopkeeper sitting zone
Worker standing zone
Customer zone
Check tablet to ensure that all monitors are on and recording data
Ensure that all Aranet4’s are recording data at 1 minute intervals
Release a bolus of CO2
Explain to market workers that you will be releasing a small amount of carbon dioxide that is not harmful at the level you will release it - you would have to release five times more for it to be risky
Bring fire extinguisher to area in front of chicken holding area
Release CO2 from fire extinguisher for 1 puff
We ideally would clear out the shop for this component but that may not be possible.
Use cardboard or other material to fan room to ensure mixing for 1 minute
Turn on ventilation fan
Check on the tablet that the CO2 levels have reached 3000 ppm
Record time bolus was released in data collection form
If the test specifies that the intervention should be “on” for this experiment, turn on the intervention
Allow Aranet4 monitors to continue running while rest of samples are being collected
Draw out where the Aranet4 monitors are placed in the sketch of the shop
At end of sampling period (1 hour)
Confirm on the application that the CO2 levels have returned to steady state (that they are similar to what they were before bolus was added)
If it has not allow the monitors to continue running
Remove the Aranet 4’s and place them in Ziplock bag within the supplies backpack
Record the time on tablet that the Aranets were removed
Download data from sampling run
Follow these steps to export data from sensor and save CSV on project box as
CO2_[Market#]_[LOCATION]_[DATE].CSV
Calibrate Aranet 4 monitors once a month by flipping the switch on their back on and off.