Sep 21, 2025

Public workspaceLB broth

DOI
https://dx.doi.org/10.17504/protocols.io.q26g7n35klwz/v1
  • Jordan Ward1
  • 1UC, Santa Cruz
Jordan Ward
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DOI: https://dx.doi.org/10.17504/protocols.io.q26g7n35klwz/v1
Protocol Citation: Jordan Ward 2025. LB broth. protocols.io https://dx.doi.org/10.17504/protocols.io.q26g7n35klwz/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: September 19, 2025
Last Modified: September 21, 2025
Protocol Integer ID: 227755
Keywords: lb broth recipe, lb of lb broth, lb broth, lb, larger volume
Funders Acknowledgements:
NIH
Grant ID: R35GM158317
Abstract
Recipe for 1 LB of LB broth. To make larger volumes scale accordingly.
Troubleshooting
Weigh 10 g tryptone, 10 g sodium chloride (NaCl) and 5 g yeast extract and add to 975 ml milliQ water in a beaker with a magnetic stir bar. Mix until powder is dissolved.
Adjust pH to ~7.0 with 10N sodium hydroxide (~ 400 µl).
Pour into a graduated cylinder and bring to 1L with milliQ water.
Transfer to 10x100 µl bottles or 2x500 ml bottles. Add a loosely tightened lid or cover with aluminum foil. Add autoclave tape and autoclave using a liquid cycle (55 minutes).