Jun 22, 2023

LB Agar

DOI
https://dx.doi.org/10.17504/protocols.io.dm6gp38xjvzp/v1
  • 1Sheffield iGEM
sgoodwin
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DOI: https://dx.doi.org/10.17504/protocols.io.dm6gp38xjvzp/v1
Protocol Citation: sgoodwin 2023. LB Agar. protocols.io https://dx.doi.org/10.17504/protocols.io.dm6gp38xjvzp/v1
License: This is an open access  protocol  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: In development
We are still developing and optimizing this protocol
Created: June 22, 2023
Last Modified: June 22, 2023
Protocol  Integer ID: 83813
Keywords: lb, growth
Abstract
Protocol for producing LB agar growth medium
LB Agar
3h 11m
Add 1.5 g LB powder, 1.5 g agarose powder to conical flask

5m

Dilute with 200 mL distilled water. Ensure fully dissolved

5m
Seal conical flask with sponge bung and foil
1m
Sterilise growth medium by autoclaving
3h