Oct 06, 2020
Version 1
Lab 5 Notebook V.1
This document is a draft, published without a DOI.
- 1UCSC
- UCSC BME 22L
Document Citation: Ikenna Anigbogu 2020. Lab 5 Notebook. protocols.io https://protocols.io/view/lab-5-notebook-bmffk3jn
License: This is an open access document distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Created: September 16, 2020
Last Modified: October 06, 2020
Document Integer ID: 42183
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Abstract
Prelab
- Name and describe the 3 stages of PCR.
- Describe how gel electrophoresis is used to distinguish between DNA base pair lengths.
- What reagents are found in a PCR tube that is ready to be put into a thermocycler? What is each reagent's function in the experiment? Give a single sentence for each reagent.
Dry Lab Answers
Provide the information asked of you in the Dry lab for steps 3-6 & 8-11.
Give a picture of your gel electrophoresis results.
Post Lab
Write about a half a page on a recent article that used gel electrophoresis and give an analysis on what they did in the experiment and how gel electrophoresis played a role. Give DOI and the article's name.
Disclaimer:
The information provided on this document is intended for the educational purposes of the BME 22L laboratory course. It is worth noting that the information listed on this document is subject to change and is not finalized. Therefore, the information on this document should not be used outside of this course.