Sep 05, 2024
KAPP-Sen TMC: Placenta tissue blocks paraffin embedding
- Ramalakshmi Ramasamy1,
- Anne Marchini2,
- Tim Adams2,
- Elaine Bechtel2,
- Lesley Bechtold2
- 1The Jackson Laboratory for Genomic Medicine, Farmington, CT, USA;
- 2The Jackson Laboratory, Bar Harbor, ME, USA
- Cellular Senescence Network (SenNet) Method Development Community
- KAPP-Sen TMC
Protocol Citation: Ramalakshmi Ramasamy, Anne Marchini, Tim Adams, Elaine Bechtel, Lesley Bechtold 2024. KAPP-Sen TMC: Placenta tissue blocks paraffin embedding. protocols.io https://dx.doi.org/10.17504/protocols.io.261ge51bjg47/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: September 05, 2024
Last Modified: September 05, 2024
Protocol Integer ID: 107030
Keywords: KAPP-Sen TMC
Funders Acknowledgements:
National Institute on Aging (NIA) KAPP-Sen Tissue Mapping Collaborative
Grant ID: U54 AG075941
Abstract
Placenta formalin-fixed tissue blocks paraffin embedding at JAX Bar Harbor Histology Services.
Procedure addition between sample collection at Mayo Clinic and JAX Bar Harbor Histology Services processing:
Samples were received from Mayo Clinic in 10% NBF on ice.
Formalin was replaced with 70% Ethanol and left overnight at room temperature.
Samples were shipped at room temperature to JAX Bar Harbor Histology Services for tissue processing and paraffin embedding (FFPE).
At JAX Bar Harbor Histology Services samples were embedded in paraffin (FFPE) according to: https://dx.doi.org/10.17504/protocols.io.rm7vzj9z2lx1/v1