Sep 26, 2023

JMN-MSMP Muscle Single Cell Isolation V.2

DOI
https://dx.doi.org/10.17504/protocols.io.j8nlko8xxv5r/v2
  • 1SenNet JMN-MSMP
  • Cellular Senescence Network (SenNet) Method Development Community
ccherry
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DOI: https://dx.doi.org/10.17504/protocols.io.j8nlko8xxv5r/v2
Protocol Citation: ccherry 2023. JMN-MSMP Muscle Single Cell Isolation. protocols.io https://dx.doi.org/10.17504/protocols.io.j8nlko8xxv5r/v2Version created by ccherry
License: This is an open access  protocol  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: September 26, 2023
Last Modified: September 26, 2023
Protocol  Integer ID: 88425
Keywords: SenNet JHM-MSMP, msmp muscle single cell isolation single cell isolation, single cell, jmn, cell, isolation
Abstract
Single cell isolation from muscle
Single cell isolation
Tissue was finely diced in RPMI 1640 (Gibco) with 1.6 Wunsh U/mL Liberase TL (Roche Diagnostics) and 0.2 mgl/mL deoxyribonuclease I (Roche Diagnostics).
Finely diced tissue was incubated at 37C for 45 minutes.
Digested tissues were ground subsequently through 100um and 70um strainers (Thermo Fisher Scientific).
Cells were centrifuged at 300g for 5 minutes and washed twice with 1x PBS