Sep 26, 2023

JMN-MSMP Muscle Bulk RNAseq V.2

DOI
https://dx.doi.org/10.17504/protocols.io.rm7vzxjz4gx1/v2
  • 1SenNet JMN-MSMP
  • Cellular Senescence Network (SenNet) Method Development Community
ccherry
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DOI: https://dx.doi.org/10.17504/protocols.io.rm7vzxjz4gx1/v2
Protocol Citation: ccherry 2023. JMN-MSMP Muscle Bulk RNAseq. protocols.io https://dx.doi.org/10.17504/protocols.io.rm7vzxjz4gx1/v2Version created by ccherry
License: This is an open access  protocol  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: September 26, 2023
Last Modified: September 26, 2023
Protocol  Integer ID: 88426
Keywords: SenNet JHM-MSMP, msmp muscle bulk rnaseq rna, rna, jmn, sequencing
Abstract
RNA sequencing of muscle
Bulk RNA sequencing
cDNA synthesis and library preparation were performed using the TruSeq RNA Library Prep Kit V2 following the manufacturer's protocol.
The Agilent BioAnalyzer system was used to perform quality control on samples during the library prep process as suggested in the manufacturer's protocol and RNA samples with RIN scores below 7 post-cDNA synthesis were excluded from analysis.
Libraries were pooled and sequenced to a target depth of 30M unique paired end 50bp x 50bp reads per sample using a NovaSeq 6000.