Dec 09, 2025
Jess western blot automated system
- Ellen Hertz1
- 1National Institutes of Health
Protocol Citation: Ellen Hertz 2025. Jess western blot automated system. protocols.io https://dx.doi.org/10.17504/protocols.io.q26g7nq11lwz/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: December 09, 2025
Last Modified: December 09, 2025
Protocol Integer ID: 234607
Keywords: ASAPCRN, jess western blot, samples for jess, system brief description of preparation, western blot, detailed instruction, automated system, sample, reagent pack, preparation, jess
Funders Acknowledgements:
ASAP
Grant ID: ASAP-000458
Abstract
Brief description of preparation of samples for Jess western blot automated system. Detailed instructions are included with the reagent pack.
Troubleshooting
Preparation of samples for Jess
There is a detailed instruction included in the reagent pack from Bio Techne. The protocol was applied without changes.
Open EZ Standard pack. Reconstitute DTT (40 uL dH2O), Fluorescent 5X Master Mix (20 uL 10X Sample buffer and 20 uL DTT) and Biotinylated Ladder (20 ul dH20)
Dilute 10x sample buffer to 0.1x sample buffer in dH2O
Combine one-part fluorescent master mix with four parts of sample (final concentration of 1 μg/μl)
Boil samples and biotinylated ladder at 95 °C for 5 min
Load Separation Capillary Cartridges with 3 ul of sample per well and antibodies
Spin plate at 1000 x g for 5 min
Load Separation Capillary Cartridges with wash buffer, luminol-peroxide mix and RePlex
reagent (if running replex protocol)