Nov 05, 2025
JAX-Sen: Mice Selection Criteria (Placenta)
- alicia.warren 1,
- Vicki Ingalls1,
- Susan Sheehan1
- 1The Jackson Laboratory, Bar Harbor, ME, USA
- Cellular Senescence Network (SenNet) Method Development Community
Protocol Citation: alicia.warren , Vicki Ingalls, Susan Sheehan 2025. JAX-Sen: Mice Selection Criteria (Placenta) . protocols.io https://dx.doi.org/10.17504/protocols.io.kxygx42jzl8j/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: October 15, 2025
Last Modified: November 05, 2025
Protocol Integer ID: 229780
Keywords: JAX-Sen, mouse placenta, mice selection criteria, placenta, female fetus different timepoint, female fetus, spatial assays such as xenium, bulkrna, scrna
Funders Acknowledgements:
National Institute on Aging (NIA) JAX-Sen Senescence Tissue Mapping Center
Grant ID: U54 AG079753
Abstract
We aim to select a mouse placentas each from male and female fetus different timepoints to perform scRNA-seq, bulkRNA-seq, and spatial assays such as Xenium, CODEX Phenocycler and visium and H&E.
Guidelines
This protocol requires prior approval by the users' Institutional Animal Care and Use Committee (IACUC) or equivalent ethics committee.
Troubleshooting
We aim to select a minimum of n=4 mouse placentas each from male and female fetus (total of 8) at 4 different timepoints.
For C57Bl/6 strain (E9.5, E11.5, E13.5 and E17.5) and at 1 timepoint (E13.5) for 7 other mouse strains (WSB, A/J, NOD, NZO, 129S1, PWK and CAST) that have not been studied before are used.
With these placentas, we plan to perform scRNA-seq, bulkRNA-seq, and spatial assays such as Xenium, CODEX Phenocycler and visium and H&E) for a total of ~150 placentas.