Jul 17, 2025

Public workspaceJAX-Sen: Mice selection criteria for Xenium 

DOI
https://dx.doi.org/10.17504/protocols.io.n92ld6yw8g5b/v1
  • Binsheng Wang1,
  • Lichao Wang2,
  • Pengyi Yan1,
  • Junyu Zhu1,
  • Ming Xu1
  • 1University of Minnesota;
  • 2Peking University
  • Cellular Senescence Network (SenNet) Method Development Community
Harshpreet Chandok
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DOI: https://dx.doi.org/10.17504/protocols.io.n92ld6yw8g5b/v1
Protocol Citation: Binsheng Wang, Lichao Wang, Pengyi Yan, Junyu Zhu, Ming Xu 2025. JAX-Sen: Mice selection criteria for Xenium . protocols.io https://dx.doi.org/10.17504/protocols.io.n92ld6yw8g5b/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: July 15, 2025
Last Modified: July 17, 2025
Protocol Integer ID: 222583
Keywords: JAX-Sen, mice selection criteria, xenium data, senescence in liver, xenium, senescence, mice, varying age, genotype
Abstract
We aim to select mice of varying ages and genotypes across both male and female groups to study senescence in liver, heart, kidney and muscle tissues in order to generate xenium data. 
We included 6-month-old and 27-month-old p21-Cre/+ (P) mice, as well as 27-month-old p21-Cre/+; DTA/+ (P/D) mice, generated on a C57BL/6 background. 

Guidelines
This protocol needs prior approval by the users' Institutional Animal Care and Use Committee (IACUC) or equivalent ethics committee
Troubleshooting
Safety warnings
This protocol needs prior approval by the users' Institutional Animal Care and Use Committee (IACUC) or equivalent ethics committee
Procedure
p21-Cre mice were generated on a C57BL/6 background and further crossed with floxed diphtheria toxin A (DTA) mice (The Jackson Laboratory, Bar Harbor, ME) to generate p21-Cre/+; +/+ (P) and p21-Cre/+; DTA/+ (P/D) littermates.
p21-Cre/+; LUC/+ (PL) mice were generated by crossing p21-Cre mice with floxed knock-in firefly luciferase (LUC) mice (The Jackson Laboratory).
Five 6-month-old P mice, six 27-month-old P mice, and six 27-month-old P/D mice, each group including both females and males, for a total of 17 animals were selected.