Jul 17, 2025
JAX-Sen: Collection and Shipment of Xenium samples
- Binsheng Wang1,
- Lichao Wang2,
- Pengyi Yan1,
- Junyu Zhu1,
- Ming Xu1
- 1University of Minnesota;
- 2Peking University
- Cellular Senescence Network (SenNet) Method Development Community
Protocol Citation: Binsheng Wang, Lichao Wang, Pengyi Yan, Junyu Zhu, Ming Xu 2025. JAX-Sen: Collection and Shipment of Xenium samples. protocols.io https://dx.doi.org/10.17504/protocols.io.yxmvmbxp9g3p/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: July 15, 2025
Last Modified: July 17, 2025
Protocol Integer ID: 222584
Keywords: JAX-Sen, JAX-Sen, shipment of xenium sample, xenium sample, xenium, shipment the jackson laboratory, jackson laboratory, details on specimen collection, specimen collection, jax, genomic medicine, ct for processing
Funders Acknowledgements:
The Jackson Laboratory Senescence Tissue Mapping Center (JAX-Sen TMC)
Grant ID: U54AG079753
Abstract
In this protocol we provide details on specimen collection and shipment The Jackson Laboratory for Genomic Medicine (JAX-GM) in Farmington, CT for processing of xenium samples.
Guidelines
This protocol needs prior approval by the users' Institutional Animal Care and Use Committee (IACUC) or equivalent ethics committee
Troubleshooting
Safety warnings
This protocol needs prior approval by the users' Institutional Animal Care and Use Committee (IACUC) or equivalent ethics committee
Reagents and Materials
4% paraformaldehyde
70% ethanol
Paraffin
Tamoxifen
PBS
Procedure
Collection/Harvest
27-month-old mice received two doses of tamoxifen (dissolved in corn oil) per month starting at age 20 months.
6-month-old mice received 2 mg of tamoxifen intraperitoneally daily for two consecutive days, and tissues were collected 10 days later.
Immediately after animals were euthanized using carbon dioxide, the heart, liver, muscle, and kidney were collected, briefly immersed in PBS, and then fixed in 4% paraformaldehyde for 24 h.
The samples were then transferred to a 70% ethanol solution, embedded in paraffin, and the blocks were trimmed to expose the tissue.
Sample cores were collected from paraffin blocks to construct tissue microarrays, and the tissues were sectioned into 5 μm-thick slices.
Shipping
Samples were placed in double-sealed bags and delivered in person to the Jackson Laboratory for Genomic Medicine, Farmington, CT 06032.