- Dialysis tube 12 kDa MWCO (Sigma, D9777) or Pur-A-Lyzer™ Midi Dialysis tubes 1 kDa MWCO (Sigma, PURD10005-1KT) for peptides
- Purified proteins/peptides (protein A, protein/peptide B)
- Dialysis Buffer (20 mM Tris pH 8.0 at 4ºC, 150 mM NaCl, 1 mM DTT)
- Calorimeter ITC 2000 (MicroCal)
- PC with the MicroCal ITC200 software (version 1.30, Malvern) and Origin 7 (OriginLab Corporation)
- Tabletop microcentrifuge (VWR MicroStar 12)