May 15, 2024
Version 3
Isolation of Nuclei from Adult Human Brain Tissue for 10x Genomics Platform V.3
Version 1 is forked from Isolation of Nuclei from Adult Human Brain Tissue for 10x Genomics Platform
- Allen Institute for Brain Science1
- 1Allen Institute
- Allen Institute for Brain ScienceTech. support
Protocol Citation: Allen Institute for Brain Science 2024. Isolation of Nuclei from Adult Human Brain Tissue for 10x Genomics Platform. protocols.io https://dx.doi.org/10.17504/protocols.io.yxmvmkerng3p/v3Version created by Allen Institute
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: May 15, 2024
Last Modified: May 15, 2024
Protocol Integer ID: 99886
Keywords: microdissected brain tissue sections for rna, frozen adult human brain tissue, nuclei from frozen adult, adult human brain tissue, human brain tissue, microdissected brain tissue section, isolation of nuclei, 10x genomics platform isolation, brain tissue, rna, nuclei, tissue
Abstract
Isolation of nuclei from frozen adult human brain tissue or thawed and microdissected brain tissue sections for RNA-seq analysis.
Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U01MH114812. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
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