This protocol is intended for isolation of Klebsiella strains from human or animal fecal material. It is derived from the initial description of the SCAi medium (van Kregten E, Westerdaal, N. A. C., and Willers, J. M. N. New, simple medium for selective recovery of Klebsiella pneumoniae and Klebsiella oxytoca from human feces. Journal of Clinical Microbiology. 1984;20:936-41) and its validation across a diversity of Klebsiella strains (Passet V, Brisse S. 2015. Association of tellurite resistance with hypervirulent clonal groups of Klebsiella pneumoniae. J Clin Microbiol. 53(4):1380-2). The protocol entails enrichment using ampicillin-containing broth, and plating on SCAi (Simmons Citrate with Inositol) agar.