Oct 09, 2022

Public workspaceIPTG_induction

DOI
https://dx.doi.org/10.17504/protocols.io.4r3l2od1pv1y/v1
IPTG_induction
  • Ana Belem García González1,
  • Georgina Diego1,
  • Jair Alexis Gardea Sáenz1,
  • Irán Alessandra Chaparro Rodríguez1
  • 1Tecnologico de Monterrey Campus Chihuahua
Denisse Chacón Ramírez
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DOI: https://dx.doi.org/10.17504/protocols.io.4r3l2od1pv1y/v1
Protocol Citation: Ana Belem García González, Georgina Diego, Jair Alexis Gardea Sáenz, Irán Alessandra Chaparro Rodríguez 2022. IPTG_induction. protocols.io https://dx.doi.org/10.17504/protocols.io.4r3l2od1pv1y/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: In development
We are still developing this protocol
Created: August 08, 2022
Last Modified: October 09, 2022
Protocol Integer ID: 68367
Keywords: using iptg, protein expression
Abstract
Protocol to induce protein expression using IPTG.
Troubleshooting
Protocol
5h
From a relatively fresh plate, grab a colony and grow DurationOvernight at Temperature37 °C andShaker225 rpm in Amount10 mL of LB+CAM.

Inoculate Amount3 mL in Amount30 mL of LB+CAM and incubate at Temperature37 °C and Shaker225 rpm

When the cells reach to a OD600 between 0.4 and 0.8, the protein expression must be induced (IPTG concentrations can vary from 0.1 to 1M).
Incubate a Temperature37 °C at Shaker225 rpm for Duration05:00:00 (Hours can vary).

5h