Oct 09, 2022

IPTG_induction

DOI
https://dx.doi.org/10.17504/protocols.io.4r3l2od1pv1y/v1
IPTG_induction
  • 1Tecnologico de Monterrey Campus Chihuahua
Denisse Chacón Ramírez
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DOI: https://dx.doi.org/10.17504/protocols.io.4r3l2od1pv1y/v1
Protocol Citation: Ana Belem García González, Georgina Diego, Jair Alexis Gardea Sáenz, Irán Alessandra Chaparro Rodríguez 2022. IPTG_induction. protocols.io https://dx.doi.org/10.17504/protocols.io.4r3l2od1pv1y/v1
License: This is an open access  protocol  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: In development
We are still developing this protocol
Created: August 08, 2022
Last Modified: October 09, 2022
Protocol  Integer ID: 68367
Keywords: using iptg, protein expression
Abstract
Protocol to induce protein expression using IPTG.
Protocol
5h
From a relatively fresh plate, grab a colony and grow Overnight at 37 °C and225 rpm in 10 mL of LB+CAM.

Inoculate 3 mL in 30 mL of LB+CAM and incubate at 37 °C and 225 rpm

When the cells reach to a OD600 between 0.4 and 0.8, the protein expression must be induced (IPTG concentrations can vary from 0.1 to 1M).
Incubate a 37 °C at 225 rpm for 05:00:00 (Hours can vary).

5h