Jan 18, 2026
iPSC karyotyping
Forked from a private protocol
- Yu Chen1
- 1National Human Genome Research Institute
Protocol Citation: Yu Chen 2026. iPSC karyotyping. protocols.io https://dx.doi.org/10.17504/protocols.io.36wgq13pkvk5/v1
Manuscript citation:
Bidirectional regulation of glycoprotein nonmetastatic melanoma protein B by β-glucocerebrosidase deficiency in GBA1 isogenic dopaminergic neurons from a patient with Gaucher disease and parkinsonism
Chase Chen, Charis Ma, Richard Sam, Jens Lichtenberg, Tiffany Chen, Ying Hao, Ziyi Li, Isabelle Kowal, Kate Andersh, Yue Andy Qi, Gani Perez, Ellen Hertz, Yan Li, Darian Williams, Mark J. Henderson, Morgan Park, Xuntian Jiang, Pilar Alvarez Jerez, Cornelis Blauwendraat, Ellen Sidransky, Yu Chen.
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: January 18, 2026
Last Modified: January 18, 2026
Protocol Integer ID: 238866
Keywords: ASAPCRN, human pluripotent stem cell, sample preparation, standard procedure, standard karyotyping service, ipsc, protocol
Funders Acknowledgements:
Aligning Science Across Parkinson's
Grant ID: ASAP-000458
Abstract
This protocol describe how iPSCs were prepared for standard Karyotyping service
Materials
| A | B | C | |
| Item | Vendor | Catalog # | |
| StemFlex medium | Thermo Fisher | A3349401 | |
| Y-27632 | R&D Systems | 1254 | |
| Colcemid | Roche | 10295892001 |
Troubleshooting
iPSC Preparation for Karyotyping
30m
Wash iPSCs with PBS
Dissociate iPSC with Accutase 4-5 min at 37 C
Count cells and seed at 1x106 cells/well in 6 well plates in StemFlex with 10 μM Y-27632
Incubate 24 hours at 37 C
Treat cells with Colcemid (1:100, Roche, 10295892001) for 12 hours.
Treated iPSCs were transferred to NHGRI Cytogenetics Core for karyotyping.
30m