This is a short tutorial on how to get started with gene prediciton in Prokaryotic genomes via the command line.Code is intended for use on an Ubuntu 16.04 LTS OS.The tools we will use here are:Prodigal. https://github.com/hyattpd/ProdigalBarrnap. https://github.com/tseemann/barrnapThis tutorial is intended for teaching purposes, but if you use any of the tools in a scientific paper do not forget to cite the appropriate publications!Prodigal:Prodigal: prokaryotic gene recognition and translation initiation site identification.BMC Bioinformatics, 2010, Volume 11, Number 1, Page 1Doug Hyatt, Gwo-Liang Chen, Philip F LoCascio, Miriam L Land, Frank W Larimer, Loren J Hauser Barrnap:Seemann T (2013), barrnap 0.8 : rap. id ribosomal RNA predictionhttps://github.com/tseemann/barrnapBedtools BEDTools: a flexible suite of utilities for comparing genomic features. Bioinformatics, Volume 26, Issue 6, 15 March 2010, Pages 841–842, https://doi.org/10.1093/bioinformatics/btq033