Nov 20, 2025

Public workspaceInduction of Sucrosomes

DOI
https://dx.doi.org/10.17504/protocols.io.14egnry66l5d/v1
  • Devin Clegg1,
  • Shawn Ferguson2,3,4,5,6,7
  • 1Yale University;
  • 2Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06510, USA;
  • 3Neuroscience, Yale University School of Medicine, New Haven, Connecticut 06510, USA;
  • 4Program in Cellular Neuroscience, Neurodegeneration and Repair;
  • 5Wu Tsai Institute Yale University School of Medicine, New Haven, Connecticut 06510, USA;
  • 6Aligning Science Across Parkinson’s (ASAP) Collaborative Research Network, Chevy Chase, MD, 20815, USA;
  • 7Kavli Institute for Neuroscience, Yale University School of Medicine, New Haven, Connecticut 06510, USA
  • Devin Clegg: Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06510, USA; Neuroscience, Yale University School of Medicine, New Haven, Connecticut 06510, USA; Program in Cellular Neuroscience, Neurodegeneration and Repair; Wu Tsai Institute Yale University School of Medicine, New Haven, Connecticut 06510, USA; Aligning Science Across Parkinson’s (ASAP) Collaborative Research Network, Chevy Chase, MD, 20815, USA;
Devin Clegg
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DOI: https://dx.doi.org/10.17504/protocols.io.14egnry66l5d/v1
Protocol Citation: Devin Clegg, Shawn Ferguson 2025. Induction of Sucrosomes. protocols.io https://dx.doi.org/10.17504/protocols.io.14egnry66l5d/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: October 16, 2025
Last Modified: November 20, 2025
Protocol Integer ID: 229999
Keywords: induction of sucrosome, sucrosome, enlarged lysosome, supplementing sucrose, sucrose in media
Funders Acknowledgements:
Aligning Science Across Parkinson’s Disease
Grant ID: ASAP-025173
Abstract
Supplementing sucrose in media to induce enlarged lysosomes/sucrosomes
Troubleshooting
Day 1
Plate 5.0 x 105 RAW 264.7 cells per well in a 6-well dish.
Day 2
16h
Remove media and wash with 1x PBS
Incubate cells with Amount2 mL of DMEM media supplemented with Concentration50 millimolar (mM) sucrose for Duration16:00:00 at Temperature37 °C .

16h