A hallmark of legume plants is their ability to establish mutualistic symbioses with Rhizobium bacteria and arbuscular mycorrhizal fungi (Rhizophagus irregularis). These symbionts help in providing nutritional benefits to the host plants. Herein, we provide a protocol to inoculate the common bean for the induction of symbiont colonization. This protocol has been routinely used in our laboratory with high reproducibility.