Jun 22, 2023

Induced Pluripotent Stem Cell Reagents

DOI
https://dx.doi.org/10.17504/protocols.io.rm7vzxnorgx1/v1
  • 1Jason Waligorski, Collin Kremitzski, Purva Patel
Mallory Wright
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DOI: https://dx.doi.org/10.17504/protocols.io.rm7vzxnorgx1/v1
Document Citation: wmallory 2023. Induced Pluripotent Stem Cell Reagents. protocols.io https://dx.doi.org/10.17504/protocols.io.rm7vzxnorgx1/v1
License: This is an open access  document  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Created: June 22, 2023
Last Modified: June 22, 2023
Document  Integer ID: 83826
Keywords: induced pluripotent stem cell, pluripotent stem cell, reagent
Abstract
reagents used to maintain our IPSC


RockiSTEMCELL Technologies Inc.Catalog #72304

Note
iPSCs like being in clusters. Single cell iPSCs tend to die. ROCKi prevents that from happening

Need a 10mM stock: dilute 5mg of the powder in 1.56 ml nuclease free water. Aliquot into smaller quantities. Store in -20C.

RevitaCell is a good alternative, either can be used


mTeSR plus media kitSTEMCELL Technologies Inc.Catalog #100-0276 4 °C
Antibiotic/Antimycotic (100X)Thermo Fisher ScientificCatalog #15240062 -20 °C
ReLeSRSTEMCELL Technologies Inc.Catalog #05872 Room temperature
TrypLE Select (1X) no phenyl redThermo Fisher ScientificCatalog # 12563-011 Room temperature
Matrigel Matrix hESC qualifiedCorningCatalog #354277 -20 °C
DMEM/F12 (1:1)ThermofisherCatalog #11320033 4 °C
CryoStor CS10 -100mlSTEMCELL Technologies Inc.Catalog #07930 4 °C
DBPS -/- (without calcium / magnesium)Thermo Fisher ScientificCatalog #14190144 Room temperature