Dec 19, 2023

Public workspaceIn vivo reduction of age-dependent neuromelanin accumulation mitigates features of Parkinson’s disease V.2

DOI
dx.doi.org/10.17504/protocols.io.rm7vzbno2vx1/v2
  • 1Vall d'Hebron Research Institute
  • Vilalab Public
Marta Gonzalez-Sepulveda
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DOI: dx.doi.org/10.17504/protocols.io.rm7vzbno2vx1/v2
Collection Citation: Marta Gonzalez-Sepulveda, Joan Compte, Miquel Vila 2023. In vivo reduction of age-dependent neuromelanin accumulation mitigates features of Parkinson’s disease . protocols.io https://dx.doi.org/10.17504/protocols.io.rm7vzbno2vx1/v2Version created by Marta Gonzalez-Sepulveda
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: December 19, 2023
Last Modified: December 19, 2023
Collection Integer ID: 92485
Funders Acknowledgements:
ASAP
Grant ID: ASAP-020505
Abstract
Methodological collection for In vivo reduction of age-dependent neuromelanin accumulation mitigates features of Parkinson’s disease
Files
Protocol
Icon representing the file Stereology-mediated cell count using StereoInvestigator
Name
Stereology-mediated cell count using StereoInvestigator
Version 1
,
joan.compte
Protocol
Icon representing the file cDNA synthesis
Name
cDNA synthesis
Version 1
, Vall d'Hebron Research Institute
Núria PeñuelasVall d'Hebron Research Institute
Protocol
Icon representing the file Determination of free and protein-bound DA and NE and their metabolites and oxidation products by UPLC-MS/MS method
Name
Determination of free and protein-bound DA and NE and their metabolites and oxidation products by UPLC-MS/MS method
Version 1
,
Marta Gonzalez-Sepulveda
Protocol
Icon representing the file Gene expression analysis by quantitative Real-Time PCR (qPCR)
Name
Gene expression analysis by quantitative Real-Time PCR (qPCR)
Version 1
, Vall d'Hebron Research Institute
Núria PeñuelasVall d'Hebron Research Institute
Protocol
Icon representing the file RNA and protein extraction from bulk dissections
Name
RNA and protein extraction from bulk dissections
Version 1
, Vall d'Hebron Research Institute
Núria PeñuelasVall d'Hebron Research Institute
Protocol
Icon representing the file Immunochemistry on paraffin sections
Name
Immunochemistry on paraffin sections
Version 1
, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP
Protocol
Icon representing the file Immunofluorescence on paraffin sections
Name
Immunofluorescence on paraffin sections
Version 1
, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP
Protocol
Icon representing the file Rat brain processing for histological analyses (update)
Name
Rat brain processing for histological analyses (update)
Version 2
, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP
Protocol
Icon representing the file Stereotaxic rat brain surgery for Substantia Nigra targeting
Name
Stereotaxic rat brain surgery for Substantia Nigra targeting
Version 1
, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP
Protocol
Icon representing the file Cylinder behavioral test
Name
Cylinder behavioral test
Version 1
,
joan.compte
Protocol
Icon representing the file Intracellular neuromelanin quantification
Name
Intracellular neuromelanin quantification
Version 1
, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP