Dec 19, 2023

In vivo reduction of age-dependent neuromelanin accumulation mitigates features of Parkinson’s disease V.2

DOI
https://dx.doi.org/10.17504/protocols.io.rm7vzbno2vx1/v2
  • 1Vall d'Hebron Research Institute
  • Vilalab Public
Marta Gonzalez-Sepulveda
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DOI: https://dx.doi.org/10.17504/protocols.io.rm7vzbno2vx1/v2
Collection Citation: Marta Gonzalez-Sepulveda, Joan Compte, Miquel Vila 2023. In vivo reduction of age-dependent neuromelanin accumulation mitigates features of Parkinson’s disease . protocols.io https://dx.doi.org/10.17504/protocols.io.rm7vzbno2vx1/v2Version created by Marta Gonzalez-Sepulveda
License: This is an open access  collection  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: December 19, 2023
Last Modified: December 19, 2023
Collection  Integer ID: 92485
Keywords: dependent neuromelanin accumulation mitigates features of parkinson, dependent neuromelanin accumulation mitigates feature, vivo reduction of age, parkinson
Funders Acknowledgements:
ASAP
Grant ID: ASAP-020505
Abstract
Methodological collection for In vivo reduction of age-dependent neuromelanin accumulation mitigates features of Parkinson’s disease
Files
Protocol
Name
Stereology-mediated cell count using StereoInvestigator
Version 1
Created by
Image of joan.compte
joan.compte
Protocol
Name
cDNA synthesis
Version 1
Created by
Image of Núria Peñuelas, Vall d'Hebron Research Institute
Núria PeñuelasVall d'Hebron Research Institute
Protocol
Name
Determination of free and protein-bound DA and NE and their metabolites and oxidation products by UPLC-MS/MS method
Version 1
Created by
Image of Marta Gonzalez-Sepulveda
Marta Gonzalez-Sepulveda
Protocol
Name
Gene expression analysis by quantitative Real-Time PCR (qPCR)
Version 1
Created by
Image of Núria Peñuelas, Vall d'Hebron Research Institute
Núria PeñuelasVall d'Hebron Research Institute
Protocol
Name
RNA and protein extraction from bulk dissections
Version 1
Created by
Image of Núria Peñuelas, Vall d'Hebron Research Institute
Núria PeñuelasVall d'Hebron Research Institute
Protocol
Name
Immunochemistry on paraffin sections
Version 1
Created by
Image of Miquel Vila, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP
Protocol
Name
Immunofluorescence on paraffin sections
Version 1
Created by
Image of Miquel Vila, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP
Protocol
Name
Rat brain processing for histological analyses (update)
Version 2
Created by
Image of Miquel Vila, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP
Protocol
Name
Stereotaxic rat brain surgery for Substantia Nigra targeting
Version 1
Created by
Image of Miquel Vila, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP
Protocol
Name
Cylinder behavioral test
Version 1
Created by
Image of joan.compte
joan.compte
Protocol
Name
Intracellular neuromelanin quantification
Version 1
Created by
Image of Miquel Vila, VHIR-CIBERNED-ASAP
Miquel VilaVHIR-CIBERNED-ASAP