Aug 05, 2020

Public workspaceIn-silico analysis

  • 1domnic colvin
  • UM-DAE Centre for Excellence in Basic Sciences
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Document Citationavinash.kale 2020. In-silico analysis. protocols.io https://dx.doi.org/10.17504/protocols.io.bjdkki4w
License: This is an open access document distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Created: August 05, 2020
Last Modified: August 05, 2020
Document Integer ID: 40076
In-silico analysis
The sequences of mosquito larvicidal toxins reported for B. thuringiensis like all Crystal (Cry1 to Cry73) proteins and Vegetative insecticidal proteins (VIP-1, VIP-2, VIP-3, VIP-4) in addition to the Binary toxins (Bin-A and Bin-B) and Mosquito-cidal (Mtx-1; Mtx-2; Mtx-3) from B. sphaericus, were used as template sequences to search for homologus sequences in the NCBI-BLAST analysis. out against the databases of Bacillus paramycoides, Bacillus australimaris, Bacillus cereus, and Bacillus tequilensis and genome by using p-BLAST (https://blast.ncbi.nlm.nih.gov) with default search parameters.