Nov 20, 2020

Public workspaceImporting live fungi to the US (UF Forest Entomology Lab)

Importing live fungi to the US (UF Forest Entomology Lab)
  • Demian F Gomez1,
  • You Li1,
  • Jiri Hulcr1
  • 1University of Florida
  • Protocols Bark Beetle Mycobiome
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Document CitationDemian F Gomez, You Li, Jiri Hulcr 2020. Importing live fungi to the US (UF Forest Entomology Lab). protocols.io https://dx.doi.org/10.17504/protocols.io.bnuymexw
License: This is an open access document distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Created: October 23, 2020
Last Modified: November 20, 2020
Document Integer ID: 43640
Keywords: united states for the uf forest entomology lab, importing live fungi, uf forest entomology lab, live fungi to the me, live fungi through airport custom, bark beetle mycobiome, part of the bark beetle mycobiome, live fungi, fungus symbiosis, fungi, fungus, widespread insect, more information on the bbm, symbiosis, airport custom
Abstract
This protocol describes how to hand-carry live fungi through airport customs into the United States for the UF Forest Entomology Lab.

This protocol is part of the Bark Beetle Mycobiome (BBM) Research Coordination Network. For more information on the BBM international network: Hulcr J, Barnes I, De Beer ZW, Duong TA, Gazis R, Johnson AJ, Jusino MA, Kasson MT, Li Y, Lynch S, Mayers C, Musvuugwa T, Roets F, Seltmann KC, Six D, Vanderpool D, & Villari C. 2020. Bark beetle mycobiome: collaboratively defined research priorities on a widespread insect-fungus symbiosis. Symbiosis 81: 101–113 https://doi.org/10.1007/s13199-020-00686-9.
These are the conditions on our USDA permit for hand-carrying live fungi through airport customs into the United States. All fungi have to be on minislants.

1. Notify PPQ Permit Compliance Officer by email ([email protected]) to provide specific information on the trip and who is travelling. The Compliance Officer will notify Customs and Border Protection (CBP) Agriculture Specialist at the port of entry to facilitate the entry.
2. Indicate that the samples are being imported under a USDA permit on the Customs Declaration form.
3. Show CBP officers the following: Passport or Visa and a valid hand carry PPQ Form 599 Red/White label corresponding to the permit.
4. Samples need to be securely packaged as the permit conditions. Usually,  seal the minislants and the slant box. 
5. After CBP confirmation and clearance through the first port of entry into the United States, samples must be transported directly to the facility authorized in the permit. Do not preserve it in the other places (such as hotel and personal locality)
6. Upon arrival at the facility, the PPQ Compliance Officer must be notified within 24 hours that the organisms arrived. Notification may be by fax (301-734-5392) or email ([email protected]), by an independent third party (e.g. containment facility director, departmental chair, campus biosafety officer, etc.). Include the permit number, label number, date of arrival, the specific organisms that were imported, their origin, and quantity. 
7. Only people listed in the permit are authorized to carry samples.
8. An authorization to hand-carry includes only the organism in the permit.