Jan 05, 2023

Public workspaceImmunostaining infiltrating spheroids as preparation for quantitative light-sheet imaging V.2

PLOS One
Peer-reviewed method
DOI
https://dx.doi.org/10.17504/protocols.io.eq2ly77krlx9/v2
  • Benedicte Bjørknes1,
  • Oliver Emil Neye1,
  • Petra Hamerlik2,3,
  • Liselotte Jauffred1
  • 1The Niels Bohr Institute, University of Copenhagen, Blegdamsvej 17, DK-2100 Copenhagen O, Denmark;
  • 2Danish Cancer Society, Strandboulevarden 49, 2100 Copenhagen Denmark;
  • 3Division of Cancer Sciences, University of Manchester, M13 9NT Manchester, United Kingdom
Liselotte Jauffred
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DOI: https://dx.doi.org/10.17504/protocols.io.eq2ly77krlx9/v2
Protocol Citation: Benedicte Bjørknes, Oliver Emil Neye, Petra Hamerlik, Liselotte Jauffred 2023. Immunostaining infiltrating spheroids as preparation for quantitative light-sheet imaging. protocols.io https://dx.doi.org/10.17504/protocols.io.eq2ly77krlx9/v2Version created by Liselotte Jauffred
Manuscript citation:
PLOS2022 this protocol is associated with a PLOS ONE Lab Protocol Submission with the title: "Immunostaining protocol for infiltrating brain cancer spheroids for light-sheet imaging" with manuscript number of our PLOS ONE submission: PONE-D-22-31029
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: January 05, 2023
Last Modified: January 05, 2023
Protocol Integer ID: 74805
Keywords: glioblastoma, invasion assay, immunostaining, hydrogels, spheroids, tumorospheres, light-sheet microscopy, 3d human xenograft glioblastoma spheroid model, studying glioblastoma cell invasion, immunostaining infiltrating spheroid, glioblastoma cell invasion, glioblastoma biology, key features of glioblastoma biology, using indirect immunofluorescence, infiltrating spheroids as preparation, vitro model, various in vivo, cell, sheet imaging, imaging
Funders Acknowledgements:
Independent Research Fund Denmark
Grant ID: DFF 0165-00032B
Independent Research Fund Denmark
Grant ID: DFF 0165-00103B
Abstract
Although various in vivo and in vitro models for studying glioblastoma cell invasion has progressed the field, there is still a need for optimized procedures. In particular to reveal key features of glioblastoma biology and infiltrating growth. In this protocol, we present an approach using indirect immunofluorescence in a 3D human xenograft glioblastoma spheroid model embedded in a naturally derived extracellular matrix
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