Sep 27, 2024
Immunopeptidomic analyses of RAW 264.7 macrophages
- Joel Lanoix1,2,
- Pierre Thibault1,2
- 1Institute for Research in Immunology and Cancer, Université de Montréal, Montreal, Quebec, Canada;
- 2Aligning Science Across Parkinson’s (ASAP) Collaborative Research Network, Chevy Chase, MD, 20815
Collection Citation: Joel Lanoix, Pierre Thibault 2024. Immunopeptidomic analyses of RAW 264.7 macrophages. protocols.io https://dx.doi.org/10.17504/protocols.io.rm7vzxbergx1/v1
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: August 09, 2023
Last Modified: September 27, 2024
Collection Integer ID: 86250
Keywords: ASAPCRN, immunopeptidomic analyses of raw, immunopeptidomic analysis, associated peptide, peptides from raw, macrophage, peptide, antigen, mass spectrometry, novel proteogenomic approach, cells after lps stimulation
Funders Acknowledgements:
Aligning Science Across Parkinson’s
Grant ID: ASAP 000525
Disclaimer
ETHICS DISCLAIMER
The protocols.io team notes that research involving animals and humans must be conducted according to internationally-accepted standards and should always have prior approval from an Institutional Ethics Committee or Board.
Abstract
Here, we describe protocols for a novel proteogenomic approach to identify MHC I–associated peptides from RAW 264.7 cells after LPS stimulation. These antigens are rare and are most effectively identified with a mass spectrometry–based approach, which allows the direct sampling and sequencing of these peptides.
Troubleshooting
ETHICS DISCLAIMER
The protocols.io team notes that research involving animals and humans must be conducted according to internationally-accepted standards and should always have prior approval from an Institutional Ethics Committee or Board.
Files
MAPs isolation from mouse cells.docx
M&M_MHC-1 peptide isolation.docx
Anti H2-Db antibody.docx
Solubilization of mouse cells.docx
Pan-H2, H2-Kb, H2-KdDd antibodies.docx