This staining was employed to detect Fetuin-A\u00a0 in paraffin sections (1 \u03bcm thickness) of formalin-fixed human brain tissue. For fetuin-A staining, we used a monoclonal IgG2a mouse-anti-human antibody (clone MAHS-1, dilution 0.1-0.5 \u00b5g\/mL), raised against purified human fetuin-A in our laboratories. Antibodies were diluted in a 1% dilution of Bovine serum albumin (BSA) in phosphate-buffered saline (PBS) and were immediately applied to the re-hydrated sections. Bound antibody was detected using Dako REAL\u2122 Detection System, which employs APAAP immunochemistry and fast red chromogenic substrate (Dako K5000, Glostrup, Denmark) following the manufacturers protocol. Counterstaining was employed with Mayer\u00b4s hematoxylin solution (Roth, T160.1, one minute). The slides were then washed in demineralized water and dehydrated in graded alcohol (concentrations from 70% to 100%). After placing in xylene the sections were mounted (Roth, T160.1) and covered using coverslips.