Apr 23, 2020

Public workspaceImmediate Early Gene (IEG) mapping of spinal cord neurons activated by cystometry-induced micturition in rats [keast-002]

DOI
dx.doi.org/10.17504/protocols.io.bakxicxn
  • 1University of Melbourne
  • SPARC
    Tech. support email: info@neuinfo.org
Janet R Keast
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DOI: dx.doi.org/10.17504/protocols.io.bakxicxn
Collection Citation: Janet R Keast, Peregrine B Osborne, Nicole Wiedmann 2020. Immediate Early Gene (IEG) mapping of spinal cord neurons activated by cystometry-induced micturition in rats [keast-002]. protocols.io https://dx.doi.org/10.17504/protocols.io.bakxicxn
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: December 17, 2019
Last Modified: November 27, 2023
Collection Integer ID: 31095
Keywords: physiology, autonomic, activity-mapping, neuroanatomy, immunohistochemistry, image analysis
Abstract
This collection describes the procedures required to visualize and characterize lumbosacral spinal neurons that are activated by cystometry of awake adult male and female Sprague-Dawley rats. This collection includes protocols for:

STAGE 1: Surgery to cannulate the bladder, followed by recovery then cystometry

STAGE 2: Intracardiac perfusion with fixative to preserve the spinal cord tissue

STAGE 3: Immunohistochemical labelling of spinal cord sections to visualise immediate early gene expression in specific spinal regions and neuronal populations

STAGE 4: Microscopy and image analysis to assess patterns of immediate early gene expression in different spinal cord regions
Files
Protocol
Icon representing the file Cystometry in awake rats
Name
Cystometry in awake rats
Version 1
, University of Melbourne
Janet R KeastUniversity of Melbourne
Protocol
Icon representing the file Intracardiac perfusion with fixative for anatomical studies
Name
ForkIntracardiac perfusion with fixative for anatomical studies
Version 1
, University of Melbourne
Janet R KeastUniversity of Melbourne
Protocol
Icon representing the file Immunohistochemical labelling of spinal cord neurons involved in bladder activity
Name
Immunohistochemical labelling of spinal cord neurons involved in bladder activity
Version 1
, University of Melbourne
Janet R KeastUniversity of Melbourne
Protocol
Icon representing the file Image analysis of immediate early gene expression in spinal cord sections
Name
Image analysis of immediate early gene expression in spinal cord sections
Version 1
, University of Melbourne
Janet R KeastUniversity of Melbourne