Jul 13, 2024

Imaging- Confocal

DOI
https://dx.doi.org/10.17504/protocols.io.j8nlk88y1l5r/v1
  • 1Department of Clinical Neuroscience, Karolinska Institutet, 171 76 Stockholm, Sweden;
  • 2Aligning Science Across Parkinson’s (ASAP) Collaborative Research Network, Chevy Chase, MD 20815, USA
Jacquelyn Haytayan
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DOI: https://dx.doi.org/10.17504/protocols.io.j8nlk88y1l5r/v1
Protocol Citation: daniel.dautan daniel, Per Svenningsson 2024. Imaging- Confocal. protocols.io https://dx.doi.org/10.17504/protocols.io.j8nlk88y1l5r/v1
License: This is an open access  protocol  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: June 05, 2024
Last Modified: September 24, 2024
Protocol  Integer ID: 101201
Keywords: ASAPCRN, imaging, mouse brain, using confocal microscope, confocal microscope, confocal microcope, carl zeiss lsm 880 confocal microcope, imaging, carl zeiss lsm, microscope
Funders Acknowledgements:
Aligning Science Across Parkinson's
Grant ID: 020608
Abstract
Protocol for imaging using confocal microscope. Sections for analysis should be mounted on slides, stained for appropriate markers, and coverslipped. This protocol is using a Carl Zeiss LSM 880 confocal microcope.
Using the confocal microscope, capture images at 10x magnification using a resolution of either 1024x1024 or 2048x2048.
If needed for larger area of the brain, use tile-scanning with a 0.6 zoom factor and 10% overlap for automated reconstruction.
If acquiring z-stack images use 1-4um spacing. Use stack projection in ImageJ and exclude approximately 10% of the section's surface.
Making sure to use the same settings across all images for the same experiment, use ImageJ to process the images.