Apr 02, 2020
Image J Nerve Density Percentage Analysis
- 1[ETSU Research Lab Tech]
- SPARCTech. support email: info@neuinfo.org
Protocol Citation: Elizabeth Smith, Jacqui Potter 2020. Image J Nerve Density Percentage Analysis. protocols.io https://dx.doi.org/10.17504/protocols.io.beh4jb8w
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: April 01, 2020
Last Modified: April 02, 2020
Protocol Integer ID: 35100
Abstract
ImageJ Software can be used to find the percentage of nerve density in regions of interest. These regions of interest are imaged, and the nerve percentage is found by adjusting the image's histogram threshold.
Dowload ImageJ Software
Open Image
File>Open>Locate desired image file>Click desired individual image (JPEG or TIFF)
Prepare Image for Analysis
Image>Type>8-bit
Remove over exposed areas, scale bar etc. by clicking on desired shape in Shape Tool Bar under File Tool Bar.
Analyze Image
Image>Adjust>Threshold
Move both slider bars to the far right. Slowly move the top slider bar to the left until you just fill in the areas of interest with "red". The percentage that appears under the histogram is the percentage of area filled. That is the nerve density percentage.
Reset the threshold slider bars as many times as desired. Typically, three data points which are then averaged.