Feb 04, 2026

Identification of inhibitory midbrain neurons in FFPE human midbrain sections V.1

DOI
https://dx.doi.org/10.17504/protocols.io.dm6gp153pgzp/v1
  • 1Brain and Mind Centre & Faculty of Medicine and Health School of Medical Sciences, The University of Sydney, Sydney, NSW 2050, Australia;
  • 2Aligning Science Across Parkinson’s (ASAP) Collaborative Research Network, Chevy Chase, MD, 20815
Anastasia Filimontseva
Icon indicating open access to content
QR code linking to this content
DOI: https://dx.doi.org/10.17504/protocols.io.dm6gp153pgzp/v1
Collection Citation: Anastasia Filimontseva, YuHong Fu, Glenda Halliday 2026. Identification of inhibitory midbrain neurons in FFPE human midbrain sections. protocols.io https://dx.doi.org/10.17504/protocols.io.dm6gp153pgzp/v1
License: This is an open access  collection  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: February 04, 2026
Last Modified: February 06, 2026
Collection  Integer ID: 242661
Keywords: ASAPCRN, identification of inhibitory midbrain neuron, inhibitory midbrain neuron, ffpe human midbrain sections protocols for standard processing, ffpe human brainstem section, human midbrain sections protocol, immunohistochemical staining for tyrosine hydroxylase, human brainstem section, tyrosine hydroxylase, immunohistochemical staining, ffpe
Funders Acknowledgements:
National Health and Medical Research Council Investigator Grant
Grant ID: 1176607
Aligning Science Across Parkinson's
Grant ID: ASAP-020505
Abstract
Protocols for standard processing of FFPE human brainstem sections and immunohistochemical staining for tyrosine hydroxylase (TH) and GAD67.
Files
Protocol
Name
Human Brain Tissue Processing
Version 1
Created by
Image of Anastasia Filimontseva, Brain and Mind Centre & Faculty of Medicine and Health School of Medical Sciences, The University of Sydney, Sydney, NSW 2050, Australia
Anastasia FilimontsevaBrain and Mind Centre & Faculty of Medicine and Health School of Medical Sciences, The University of Sydney, Sydney, NSW 2050, Australia
Protocol
Name
Immunohistochemical staining for GAD67 and TH in paraffin sections.
Version 1
Created by
Image of Anastasia Filimontseva, Brain and Mind Centre & Faculty of Medicine and Health School of Medical Sciences, The University of Sydney, Sydney, NSW 2050, Australia
Anastasia FilimontsevaBrain and Mind Centre & Faculty of Medicine and Health School of Medical Sciences, The University of Sydney, Sydney, NSW 2050, Australia