Sep 16, 2021
iBlot2--CHEM 584
- 1Brigham Young University
Protocol Citation: Ken Christensen 2021. iBlot2--CHEM 584. protocols.io https://dx.doi.org/10.17504/protocols.io.bybdpsi6
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: In development
We are still developing and optimizing this protocol
Created: September 16, 2021
Last Modified: September 16, 2021
Protocol Integer ID: 53317
Abstract
Transferring your proteins from your SDS-PAGE gel to a nitrocellulose membrane for western blot is quick and straightforward.
Guidelines
You should not touch your gel or membrane without gloves to avoid transferring protein from your fingers to your gel or blot.
Materials
iBlot2 stack
iBlot2 transfer instrument
Safety warnings
Standard lab safety guidelines should be followed.
Before start
Watch the video from the protocol.
iBlot2 Transfer
iBlot2 Transfer
Watch the video about setting up and running your transfer to nitrocellulose at the following link: https://videos.thermofisher.com/detail/video/6059971760001/how-to-perform-a-western-blot-dry-transfer-using-the-invitrogen-iblot-2-dry-blotting-system