Nov 01, 2019

Public workspaceHybridization chain reaction (HCR) protocol for tails of mouse embryos

DOI
dx.doi.org/10.17504/protocols.io.7pyhmpw
  • 1European Molecular Biology Laboratory (EMBL);
  • 2Molecular Instruments
Paul Gerald Layague Sanchez
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DOI: dx.doi.org/10.17504/protocols.io.7pyhmpw
Protocol Citation: Paul Gerald Layague Sanchez, Hidenobu Miyazawa, Molecular Instruments 2019. Hybridization chain reaction (HCR) protocol for tails of mouse embryos. protocols.io https://dx.doi.org/10.17504/protocols.io.7pyhmpw
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol in our group and it is working.
Created: September 26, 2019
Last Modified: November 01, 2019
Protocol Integer ID: 28120
Keywords: HCR, in situ hybridization
Abstract
This protocol is for the in situ hybridization chain reaction (HCR) of intact tails (somites and presomitic mesoderm, PSM) of mouse embryos. This is adapted from the in situ HCR v 3.0 protocol for whole-mount mouse embryos available in the website of Molecular Instruments (for details, please follow this link).

References
  • Choi, H., Schwarzkopf, M., Fornace, M., Acharya, A., Artavanis, G., & Stegmaier, J. et al. (2018). Third-generationin situhybridization chain reaction: multiplexed, quantitative, sensitive, versatile, robust.Development,145 (12), dev165753. doi: 10.1242/dev.165753
  • Choi, H., Calvert, C., Husain, N., Huss, D., Barsi, J., & Deverman, B. et al. (2016). Mapping a multiplexed zoo of mRNA expression.Development,143 (19), 3632-3637. doi: 10.1242/dev.140137