This is a very handy, previously published [Ref 1 and 2], rhinovirus (RV) screening and genotyping assay which I and many others have used on many sample extracts, mostly originating from acutely ill paediatric patients, spanning well over a decade's worth of collection dates.I have not confirmed that it can detect every single RV genotype but I do know that it detects many from each of the three RV species (Human rhinovirus A, Human rhinovirus B and Human rhinovirus C) as well as at least some Human enterovirus (EV) genotypes.The assay picks up EVs due to the shared genetic similarities in the 5'UTR target region.This assay produces a useful backup subgenomic sequencing target for use primarily should the 'Wisdom VP42' protocol fail to amplify a product for sequencing. It is worth noting that these primers span a target region that can produce confusing results. Recombination within the 5' end of the genome of some members of the genus Enterovirus can lead to 5'UTR sequences generated by RV-C genotypes that appear to be RV-As (see the figure below in which green RV-Cs appear in what is expected to be the red RV-A branch of the phylogenetic tree).