This is a very handy, previously published [Ref 1 and 2], rhinovirus (RV) screening and genotyping assay which I and many others have used on many sample extracts, mostly originating from acutely ill paediatric patients, spanning well over a decade's worth of collection dates.I have not confirmed that it can detect every single RV genotype but I do know that it detects many from each of the three RV species (Human rhinovirus A, Human rhinovirus B and Human rhinovirus C) as well as at least some Human enterovirus (EV) genotypes.The assay picks up EVs due to the shared genetic similarities in the 5'UTR target region.This is a handy backup subgenomic sequencing assay for use primarily should the 'Wisdom VP42' protocol fails to amplify a product for sequencing. It is worth noting that these primers span a target region that can produce confusing results. Recombination within the 5' end of the genome of some members of the genus Enterovirus can lead to 5'UTR sequences generated by RV-C genotypes that appear to be RV-As.