This version is based on a recipe (see forked version) my team and I used this assay\u00a0between 2008-2015; we dubbed it the 'Nix assay'. It targets the 5'UTR and employs quite degenerate oligos.In silico sequence alignments indicated the olignucleotides could theoretically detect aft least HPeV 1-7, 17 and 18. Further validation of this recipe is ongoing.However during a period of assay comparison, another assay (see link below), the 'Benschop assay' (J.Clin.Virol. 2008. 41(2):69-74), was found to produce\u00a0more sigmoidal and higher curves and 1-5 cycle improvements to CT values when compared among the sample sampe extract set.