I and my team used this assay between 2008-2015; we dubbed it the "Nix assay". It targets the 5'UTR and employs quite degenerate oligos.In silico sequence alignments indicated the olignucleotides could theoretically detect aft least HPeV 1-7, 17 and 18.However during a period of assay comparison, another assay (see link below), the "Benschop assay" (J.Clin.Virol. 2008. 41(2):69-74), was found to produce more sigmoidal and higher curves and 1-5 cycle improvements to CT values when compared among the sample sampe extract set.