Oct 13, 2023
Human Liver Sample Collection Protocol -- University of Minnesota TMCs
Forked from UMN SenNet Liver Collection Protocol
- Laura J Niedernhofer1,
- David A Bernlohr1,
- Steve Johnson2
- 1University of Minnesota, Minneapolis, MN USA;
- 2University of Minnesota
- Cellular Senescence Network (SenNet) Method Development Community
Protocol Citation: Laura J Niedernhofer, David A Bernlohr, Steve Johnson 2023. Human Liver Sample Collection Protocol -- University of Minnesota TMCs. protocols.io https://dx.doi.org/10.17504/protocols.io.yxmvm3prnl3p/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: October 13, 2023
Last Modified: October 13, 2023
Protocol Integer ID: 89212
Keywords: human liver sample collection protocol, bionet specimen procurement agreement, attached bionet specimen procurement agreement, umn ctsi biorepository, university of minnesota tmcs collection protocol, minnesota tmcs collection protocol, specimen procurement, liver, sample collection, specimen, laboratory
Funders Acknowledgements:
NIH
Grant ID: 5U54AG079754-02
NIH
Grant ID: 5U54AG076041-03
Abstract
Collection protocol obtained from the attached BioNet Specimen Procurement Agreement provided by the UMN CTSI Biorepository and Laboratory Services (BLS).
Tissue Procurment Agreement - 06.2023.pdf343KB
Preparation
Patient Identification: As soon as a patient is scheduled, the research team will email [email protected] a completed Specimen Procurement Request Form.
Patient Consent: Researcher consents. The original signed consent form will be placed in the patient chart and scanned into Epic. BioNet will verify consent in Epic. If the consent is not scanned in EPIC before the procedure, the researcher will provide BioNet a copy direct (e-mail or hard copy).
Collection Supplies: All supplies will be delivered to the BioNet office at least one day before the procedure and appropriately labeled with researcher name and CTSI Project #.
Collection Kit Components:
PBS (Study team)
Media: DMEM + antibiotics (Study team)
Media tubes (x3 per collection) (Study team)
10% Formalin (Bionet)
FFPE cassette x 5 (Bionet)
Specimen container (Bionet)
OCT compound (Bionet)
OCT mold x 4 (Bionet)
OCT quick freeze box (Bionet)
Liquid nitrogen vapor (Bionet)
Cryovials x 3 (Bionet)
Collection kit containing 10 ml EDTA blood tubes x 2 (Bionet)
Procurement and Processing of Liver Samples
- 2 liver biopsy cores will be collected by surgeon.
- The location of the tissue collection will be recorded by the surgeon.
- BLS will be paged by OR for immediate specimen pickup.
Core 1:
- Surgeon or OR staff will place core 1 in 10% formalin.
- BLS procurement will pick up specimen and request Bionet Research Specimen Order.
- BLS will tape a labeled FFPE cassette to the top of the specimen container.
- BLS procurement will submit specimen to BLS Histology for FFPE processing. Standard fixation period of 24-72 hours. H&E and trichrome stain requested.
Core 2:
- Surgeon or OR staff will place core 2 in specimen container and page BLS.
- BLS procurement will pick up specimen immediately and request Bionet Research Specimen Order. Short transit/processing time is critical for this specimen.
- Prepare OCT quick freeze box by adding liquid nitrogen. This must be done after the core is collected because the liquid nitrogen vapor will dissipate quickly.
- Place core 2 in one OCT mold.
- Ensure mold is labeled.
- Add OCT compound into mold containing core. Ensure no bubbles surround the tissue.
- After OCT sample is frozen, remove and transfer to -BOC freezer storage.
If liver is collected as a wedge:
- One wedge will be removed in the OR and placed in PBS.
- Split wedge in half and treat as core 1 and core 2, processing according to same instructions.