Aug 01, 2024
Human Knee Tissue Procurement, Resection and Multimodal Analyses
- Merissa Olmer1,
- Hannah Swahn1,
- Martin Lotz1
- 1Scripps Research
- Human BioMolecular Atlas Program (HuBMAP) Method Development Community
- RE-JOIN
Protocol Citation: Merissa Olmer, Hannah Swahn, Martin Lotz 2024. Human Knee Tissue Procurement, Resection and Multimodal Analyses . protocols.io https://dx.doi.org/10.17504/protocols.io.kqdg32bp1v25/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol for our tissue procurement procedures.
Created: July 31, 2024
Last Modified: August 01, 2024
Protocol Integer ID: 104368
Keywords: Human, Musculoskeletal, Knee, Tissue Procurement, Tissue Harvesting, Tissue Collection, Cartilage, Osteochondral, ACL, PCL, MCL, LCL, Meniscus, Bone, Fat Pad, Synovium, Patella, Femur
Funders Acknowledgements:
HuBMAP
Grant ID: AR078664_04
Disclaimer
Informed written consent was obtained from all tissue donors and the study was approved by the Scripps Institutional Review Board (IRB# 22-8069).
Abstract
This protocol describes the procurement of entire human knee joints, resection of joint tissues and allocation for multimodal analyses. The protocol is focused on entire knees that are recovered by Organ Procurement Organizations and parts of the protocol are also applicable for human joint tissues that are removed during surgery on the knee or other joints. For entire knees, samples of all joint tissues are resected to allow analysis of ‘the knee as an organ’.
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