This protocol was developed to dissociate (clear cell) renal cell carcinoma tumors in order to study single cell genomics using the Chromium Single Cell Gene Expression technology (10X Genomics). Starting from fresh biological material, the protocol was optimized to ensure fast and reproducible processing, both of which are very important criteria in single-cell are analyzes. This protocol allowed us to obtain biologically relevant high-quality data. It can easily be adapted according to the following optional steps: i) multiple sampling in several macroscopic areas of the tumor; ii) depletion of CD45+ cell populations; iii) and, progressive freezing of the dissociated tumor cells for biobanking.